Elaboration of a reliable strategy based on real-time PCR to characterize genetically modified plantlets and to evaluate the efficiency of a marker gene removal in grape (Vitis spp.).

@article{Costa2009ElaborationOA,
  title={Elaboration of a reliable strategy based on real-time PCR to characterize genetically modified plantlets and to evaluate the efficiency of a marker gene removal in grape (Vitis spp.).},
  author={Lorenza Dalla Costa and Ilaria Vaccari and Marco Mandolini and Lucia Martinelli},
  journal={Journal of agricultural and food chemistry},
  year={2009},
  volume={57 7},
  pages={
          2668-77
        }
}
  • Lorenza Dalla Costa, Ilaria Vaccari, +1 author Lucia Martinelli
  • Published in
    Journal of agricultural and…
    2009
  • Biology, Medicine
  • We have developed an effective strategy based on real-time PCR assay for the molecular characterization of genetically modified grape and to quantify the efficiency of a marker gene removal. This research has been implemented in Vitis vinifera cv. Brachetto plantlets where exogenes were inserted during cocultures of embryogenic calli with Agrobacterium tumefaciens carrying the chemically inducible site-specific cre/loxP pX6 vector where the expression of the cre recombinase is regulated by 17… CONTINUE READING

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