Eisosomes mark static sites of endocytosis

  title={Eisosomes mark static sites of endocytosis},
  author={Tobias C. Walther and Jason H. Brickner and Pablo S. Aguilar and Sebastian Bernales and Carlos Pantoja and Peter Walter},
Endocytosis functions to recycle plasma membrane components, to regulate cell-surface expression of signalling receptors and to internalize nutrients in all eukaryotic cells. Internalization of proteins, lipids and other cargo can occur by one of several pathways that have different, but often overlapping, molecular requirements. To mediate endocytosis, effectors assemble transiently underneath the plasma membrane, carry out the mechanics of membrane deformation, cargo selection and vesicle… 

Analysis of endocytosis at eisosomes

It is found that the uncharacterized AAA-ATPase protein Yta6 dynamically colocalizes with eisosomes and Emp70 during intracellular trafficking from plasma membrane to vacuole, implicating its essential role in maintaining the architecture of the endosomal compartment.

Lipid determinants of endocytosis and exocytosis in budding yeast.

Plasma membrane compartmentation in Saccharomyces cerevisiae

A previously unknown function of the membrane potential in the lateral organization of the plasma membrane of the arginine permease Can1 (MCC) is demonstrated and an inhomogeneous distribution of lipids in non-polarized cells could be demonstrated for the first time.

Intimate and Facultative? Regulation of Clathrin-Mediated Endocytosis by the Actin Cytoskeleton

This chapter addresses the complexity of CME regulation by actin and expands on the role of membrane compartmentalization on the spatial organization of clathrin-coated pits and on the multiplicity of differently structured regulators of actin dynamics at the coated pit.

Chapter 3: Slow diffusion, steric exclusion and protein conformation determine the localization of plasma membrane transporter

It is hypothesized that the distinct localization patterns found for integral membrane proteins in S. cerevisiae arises from a combination of slow lateral diffusion, steric exclusion and conditional trapping in membrane compartments.

Steric exclusion and protein conformation determine the localization of plasma membrane transporters

High-resolution single molecule imaging is used to observe diffusion of specific transmembrane proteins in and out of these microdomains, and propose features that dictate their inclusion/exclusion from these structures.

Vesicle trafficking from a lipid perspective

This work examines the importance of lipid signaling, membrane lipid organization and lipid metabolism for vesicle transport during exocytosis in budding yeast and finds that the essential requirement for some exocyTosis regulatory proteins can be bypassed by making simple manipulations of the lipids involved.

Reassessment of the role of plasma membrane domains in the regulation of vesicular traffic in yeast

The data suggest that the enrichment of certain plasma membrane proteins in the MCC does not regulate the rate of their endocytosis, and vesicular traffic is restricted to the M CP and the distribution of endocytic and exocytic sites within the MCP is independent of the M CC patch positions.

Analysis of sphingolipid-signaling at the plasma membrane of Saccharomyces cerevisiae

It is shown that Pkh-kinases regulate eisosome formation in response to alterations of complex sphingolipid levels in the plasma membrane, and a model how a homeostatic feedback loop may control sphingosome levels is provided to help in understanding how cells adjust processes, such as e isosome driven domain organization, endocytosis and actin organization to altered conditions.

Plasma membrane microdomains regulate turnover of transport proteins in yeast

The data suggest that release from MCC makes these proteins accessible to the endocytic machinery, and suggests that MCC represents a protective area within the plasma membrane to control turnover of transport proteins.



Actin assembly and endocytosis: from yeast to mammals.

This review focuses on progress made in elucidating the functions of the actin cytoskeleton in endocytosis and identifies several proteins that could harness forces produced during actin polymerization to facilitate specific steps in the endocytic process.

The conserved Pkh–Ypk kinase cascade is required for endocytosis in yeast

Observations demonstrate that Ypk1 acts downstream of the Pkh kinases to control endocytosis by phosphorylating components of the endocytic machinery.

Prm1p, a Pheromone-Regulated Multispanning Membrane Protein, Facilitates Plasma Membrane Fusion during Yeast Mating

The phenotype of Δprm1 mutants defines a new step in the mating reaction in which membranes are juxtaposed, possibly through a defined adherence junction, yet remain unfused, which suggests a role for Prm1p in plasma membrane fusion.

Spatial control of coated-pit dynamics in living cells

Clathrin-coated pits invaginating from the plasma membrane show definite, but highly limited, mobility within the membrane that is relaxed upon treatment with latrunculin B, an inhibitor of actin assembly, indicating that an actin-based framework may be involved in the mobility of these pits.

Distribution of Can1p into stable domains reflects lateral protein segregation within the plasma membrane of living S. cerevisiae cells

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Temporal and spatial coordination of exocytosis and endocytosis

Emerging insights into the coupling of exocytic and endocytic processes are discussed, both in the chemical synapses of neurons and in non-neuronal cells.

Sphingoid base signaling via Pkh kinases is required for endocytosis in yeast

Results suggest that Pkh1/2p kinases are part of a sphingoid base‐mediated signaling pathway that is required for the internalization step of endocytosis.