• DOI:10.1007/BF02819990
• Corpus ID: 43573163

Eighteenth Congress of theCzechoslovak Society for Microbiology of the Czechoslovak Academy of Sciences

@article{2008EighteenthCO,
  title={Eighteenth Congress of theCzechoslovak Society for Microbiology of the Czechoslovak Academy of Sciences},
  author={},
  journal={Folia Microbiologica},
  year={2008},
  volume={35},
  pages={465-556}
}

• Published 2008
• Biology
• Folia Microbiologica

S OF COMMUNICATIONS AND POSTERS About 400 Czechoslovak microbiologists participated in the 18th Congress of the Czechoslovak Society for Microbiology both by oral presentations and by posters. The Editor of Folia Microbiolo.~ica and the organizers of the Congress have been selected 238 abstracts which are dwided into five following sections according to the structure of the Society: I General microbiology p. 468 H Applied microbiology 480 1II Environmental microbiology 498 IV Medical microbiology 512 V Virology 539 These abstracts are completed with an authors' index on p. 552. 466 18th CONGRESS Vol. 35 Abbreviations of institutions occurring in the addresses of authors of the individual congress abstracts CBEV CCHV CCM CFV CHTF CHV CPl (~SAV zPI EZO FaF FDL EgO FN FNsP FVL HS IHE ILF J~.BC JEP KHS KNsP LF MB6r MFF MHS MU NCSR OHS OMRF OVS PAN PF PW SAV SLI]P SR SONZ ONZ SV~T Center for Biological and Ecological Sciences Chemical Research Center Czechoslovak Collection of Microorganisms Center for Physiological Sciences Faculty of Chemical Technology Hygiene and Nourishment Center Nutrition Chain Center Czechoslovak Academy of Sciences Czechoslovak Agriculture and Food Inspectorate Electrotechnical Testing Institute Faculty of Pharmacology Faculty of Pediatrics Institute of Physiology Faculty Hospital Faculty Hospital and Policlinic Faculty of General Medicine Hygiene Center. Institute Of Hygiene and Epidemiology Postgraduate Medical and Pharmaceutical Institute South-Bohemian Biological Center (of) Jan Evangelista Purkyn~ Regional Hygiene Center Regional Hospital and Policlinic Faculty of Medicine Institute of Microbiology Faculty of Mathematics and Physics Municipal Hygiene Center Masaryk University National Center for Scientific Researbh District Hygiene Center Oklahoma Medical Research Foundation District Veterinary Centre Polish Academy of Science Faculty of Science (Bratislava) Faculty of Science (Prague) Slovak Academy of Sciences Joint Laboratories and Production Plants Slovak Republic State Institute of National Health (Prague) State Institute of National Health (Bratislava) Slovak Technical University 1990 ABBREVIATIONS 467 OEB (JEF 0EVE 0EM 0EO 0EVM 0FHZ UK (.liCE fJMB CrMG fJMSB fJNZ UP I~PB UPJ~ (3PMD OSKVBL OSOL OTRCH O~PVPZ VLA V~,CHT V~,P V~,OO v~fJoz v~v v~z V0AB V0BVL V0K V~KVS VI3P V(.IPL vl3PP VI~CH VORV V0V VOVH V0VL Institute of Experimantal Botany Institute of Experimental Pharmacology Institute of Experimental Phytopathology and Entomology Institute of Experimental Medicine Institute of Experimental Oncology Institute of Experimental Veterinary Medicine Institute of Physiology of Farm Animals Charles University (Prague/Hradec K#ilov6/Plzefi) o r Comenius University (Bratislava) Institute of Landscape Ecology Institute of Molecular Biology Institute of Molecular Genetics Institute of Molecular and Subcellular Biology Institute of National Health Palacl~ University Institute of Soil Biology ~af,'lrik University Institute for the Care of Mother and Child Institute for State Inspection of Veterinary Biopreparations and Drugs Institute of Sera and Vaccines Institute of Tuberculosis and Respiratory Diseases Institute of Enviromental and Eploitation of Resources Military Medical Academy School of Chemical Technology Agricultural University (Nitra) Research Institute of Fruit Breeding Tree Research Institute of Ornamental Horticulture School of Veterinary Medicine Agricultural University (Prague/Brno) Research Institute. of Antibiotics and Biotransformations Research Institute of Biofactors and Veterinary Drugs Tannery Research Institute Research Institute of Fodder Industry and Services Food Research Institute Research Institute of Preventive Medicine Research Institute of Food Industry Research Institute of Rheumatic Diseases Research Institute for Plato Production Apicultural Research Institute Research Institute for Water Management Research Institute of Veterinary Medicine GENERAL MICROBIOLOGY Characterization of Plasmids f ~ m the Facultative Methylotroph M17 and Their Use in Construction of Vectors for Cloning in Methylotrophic Bacteria BRENNER V., HOLUBOVA I., BENADA O., HUBAdEK J. MB(I ~SAV, Prague The presence of cryptic plasmids was proved in three collection strains of methylotrophic bacteria isolated from soil. The smallest of these, detected in the pink M17 facultative methylotroph, was used for the construction of a vector for doning in methylotrophic bacteria. The conditions of lysis of the pink strain were optimized to obtain sufficient amounts of plasmid DNA. The restriction analysis and electron-microscopic measurement revealed the presence of two plasmids in the M17 strain cells: pWu (7.8 kb) and pWU14 (14 kb). The pWU7 plasmid was cloned into the E. coli multi-copy pK19 (Km~) plasmid and its detailed restriction map was constructed. Expression of the hsdS § Gene Controlled by Early Phage Promoter WEISEROV.A M., WEISER J., ZtNI~CH V., HtmACEK J. MBIfl ~SAV, Prague; Institute of Biochemistry and Physiology of Microorganisms, Academy of the USSR, Pushchino, USSR The restriction and modification phenotype of E. coli K12 cells is encoded by the hsdR, hsdM and h.sdS genes, q~he hsdS gene determining the restriction specificity is transcribed in the cell from the promoter of the hsdM gene. The expression level of the hsdS gene (with temperature-sensitive and tram-dominant hsdS +a mutation) cloned into pBR322 is thus very low. A BglII fragment of the phage containing the ts repressor and Pr promotor was inserted into the pVM2 plasmid. The expression of hsdS +d gene controlled by this promotor was determined by /n vivo translation m E. coli mini-cells. The maximum expression at 42 ~ corresponds to the maximum trans-dominant effect of the hsdS +d mutation. Application of Genetic Engineering in Brevibacterium T~KO J., Jucoxq~ M. OMB SAV, Bratislava Strains of nonpathogenic coryneform bacteria with an elevated production of amino acids used to be prepared mostly by classic techniques of mutagenesis and selection. Now it is feasible to increase the production of amino acids by genetic 1990 GENERAL MICROBIOLOGY 4169 engineering methods, either by cloning genes coding for the biosynthetic pathways of amino acids or by cloning genes for utilization of secondary sources of nutrients, into industrial microbial strains. A Bacillus amylase gene was transferred, using a recombinant pA-series plasmid, to L-lysine-producing Brevibacterium flavum strains. Adaptive Responses of Repair-Defective E. coli Mutants to Exposure to Alkylating Agents JANOVSKA E. Institute of Biophysics ~SA V, Bmo Adaptive response is an important error-free repair process that protects various organisms from mutagenic and carcinogenic action of small doses of alkylating agents. The kinetics of inactivation and the possibility of repair were followed in the MMS-induced, potentially lethal, lesions of E. coli strains deficient in the production of genes involved in other mechanisms of DNA repair. The inducible adaptive response was positive in the parental strain and in mutants defective in excision repair and hence is not dependent on the expression of the uvrA and uvrB genes. The repair level was lower in the recA strains requiring recombination of the defective DNA. The adaptive response was completely prevented by the pol mutation. Resistance and Tolerance of Bacteria to E Group Colicins ~MARDA J. Department of Biology, LF MU, Brno Receptor mutants of sensitive bacteria are referred to as resistant, those mutated in the mechanism of colicin transfer to the inner membrane are tolerant. The BtuB protein is the receptor of the E l E 9 colicins. Its binding epitopes for individual E colicins are not identical. The E2 and E3 colicin epitopes resemble each other significantly, while E1 colicin epitope differs from the others most. However, within a resistant culture only 10 t 10 -.2 cells survive the exposure to E colicins. Likewise, the tolerant mutants exhibit different sensitivity to individual E colicins; only 10 -3-10 -4 tolerant cells are not sensitive to their action. Mutation of tolerance usually afflicts the binding function of the receptor. "lMo New Colicins from Shigella I iol<,~,K V. Institute of Microbiology and Immunology, LF UK, Hradec Krdlovd A new colicin species was detected in serotype 1 and 8 of Shigella boydii strains which does not penetrate across a cellophane membrane, exhibits sensitivity 470 18th CONGRESS Vol. 35 to trypsin and withstands a 15-rain boiling. Another niwel colicin was found in an S. sonnei strain. We failed in obtaining the cellophane-transversing colicin in a higher amount, permitting i t smore detailed analysis. The col factors determining the formation of the two colicins are regarded as immobile. Mutants of the universal indicator E. coli K12 ROM strain resistant to the above colicins are sensitive to all known colicins. Formation and Regeneration of Protoplasts of Brevibacterium sp. M27 Mutant Strain VECERKOVA B., KONJCEK J., KONICKOVA-RADOCHOVA M., RYTIR V. MB(] ~SAV, Prague The formation of protoplasts and their subsequent regeneration is a prerequisite for transferring the genetic information by protoplast fusion as well as the transfer of recombinant DNA in bacteria. In the case of Brevibacterium sp. M27, four methods of protoplast formation were evaluated (Kaneko et aL 1979; Rytf~ et al. 1982; Santamaria et al. 1984; Sfinchez et al. 1986). The method of Ryti~ et al. (1982) was chosen for our experiments. This method was established using auxotrophic mutants, mutants resistant to antibiotics and mutants with multiple genetic markers. The formation of protoplasts reached 60 -99 %, their regeneration 17-76 %. A correlation between protoplast formation and growth rate was found in the case of the induced mutants. Mutagenesis in Coryneform Bacteria KON[CEK J., KONiCKOVA-RADOCHOVA M. MBI) ~SA V, Prague The mutation range caused by UV irradiation and N-methyl-N'-nitro-N-nitrosoguani