Egr-1 target genes in human endothelial cells identified by microarray analysis.

Abstract

Early growth response factor 1 (Egr-1) is a key transcriptional factor to mediate gene expression after vascular injury. To better understand the role of Egr-1 in vasculature, we globally profiled Egr-1 target genes in human endothelial cells using adenoviral gene transfer and Affymetrix oligonucleotide-based microarray technology. More than 300 genes regulated by >/=3-fold with Egr-1 overexpression were identified and, partially, confirmed by Northern and Western blotting, including genes for transcriptional regulators, signaling proteins, cell cycle regulatory proteins, growth factors, and cytokines. Among them, thymus-expressed chemokine (TECK) and IP-30 were dramatically induced by Egr-1, but TNFalpha-related apoptosis inducing ligand (TRAIL) was significantly repressed by Egr-1, suggesting that Egr-1 is a key mediator of inflammation and apoptosis in vascular cells. These data provide novel Egr-1 target genes and contribute to the understanding of the role of Egr-1 in vasculature.

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@article{Fu2003Egr1TG, title={Egr-1 target genes in human endothelial cells identified by microarray analysis.}, author={Mingui Fu and Xiaojun Zhu and Jifeng Zhang and Jian Liang and Yiming Lin and Luning Zhao and Markus U. Ehrengruber and Y. Eugene Chen}, journal={Gene}, year={2003}, volume={315}, pages={33-41} }