Egg-marking pheromones in honey-bees Apis mellifera

  title={Egg-marking pheromones in honey-bees Apis mellifera},
  author={Benjamin P. Oldroyd and Francis L. W. Ratnieks and Theresa C. Wossler},
  journal={Behavioral Ecology and Sociobiology},
they take exception to our citing their work (KatzavGozansky et al. 1997) in stating that the “chemical composition of Dufour’s gland secretions differs between queens and normal laying workers” (Oldroyd and Ratnieks 2000). Second, they point out that the Dufour’s gland may not be the site of synthesis of the hypothesised egg-marking pheromone, and indeed that this pheromone may not be secreted by the Dufour’s gland. Here we argue that: (1) there is strong evidence that a queen-produced egg… 

Preservation and loss of the honey bee (Apis) egg-marking signal across evolutionary time

It is shown that Apis mellifera workers can distinguish queen-laid from worker-l laid eggs of the dwarf honey bee A. florea, a phylogenetically distant species that diverged from the A. mellifiera lineage 6–10 mya, but workers are unable to distinguish worker- laid eggs of A. cerana, a much more recent divergence.

Do rebel workers in the honeybee Apis mellifera avoid worker policing?

Comparing the survival of three classes of eggs, namely, those laid by normal workers, rebel workers, and the queen, shows that in a queenright colony, eggs laid by rebel workers do not avoid policing.

Egg viability and worker policing in honey bees.

It is shown that in queenright honey bee colonies (Apis mellifera), worker reproduction is low, and that this difference in quality is the case for honey bees.

Apis florea and Apis cerana workers do not discriminate between queen-laid and worker-laid Apis mellifera eggs

It is shown that A. mellifera cannot distinguish queen- and worker-laid eggs of the more closely related species Apis cerana and A. florea workers do not distinguishQueen-lied from worker- laid eggs from A. Mellifera, even though A. cerana (but not A.Florea) tolerate A. tranquillis eggs for extended periods.

Mimicry of queen Dufour's gland secretions by workers of Apis mellifera scutellata and A. m. capensis

Multivariate analysis of the secretion profiles indicated that laying workers of both races mimic queens, and the secretions of the A. m.

Different policing rates of eggs laid by queenright and queenless anarchistic honey-bee workers (Apis mellifera L.)

It is shown that eggs laid by queenless anarchistic workers do not escape policing and have very similar removal rates to worker-laid eggs from queenless wild-type (i.e. non-anarchistic) colonies.

Differential reproductive success among subfamilies in queenless honeybee (Apis mellifera L.) colonies

It is shown that, first, subfamilies vary in the speed with which they activate their ovaries after queen-loss and, second, that the survival of eggs to the larval stage is unequal among subfam Families suggesting that some subfam families lay eggs that are more acceptable than others.

Reproduction and signals regulating worker policing under identical hormonal control in social wasps

JH is identified as a key regulator of both reproduction and the production of egg marking pheromones that mediate policing behaviour in eusocial wasps, and laid more eggs and the chemical profiles of their eggs were more queen-like.

The Cape honeybee phenomenon: the sympatric evolution of a social parasite in real time?

The Cape honeybee may constitute a unique subject for studying sympatric speciation of a social parasite and set the stage for the evolution of a queenless social parasitic honeybee.

Higher removal rate of eggs laid by anarchistic queens—a cost of anarchy?

The removal rate of eggs laid by anarchistic queens in standard worker-policing bioassays is determined and it is postulate that higher removal rates of queen-laid eggs due to recognition errors may be one reason that anarchy is rare in natural honeybee populations.



Evidence for a queen-produced egg-marking pheromone and its use in worker policing in the honey bee

Queen-laid eggs treated with the polar solvents methanol and ethanol were removed more rapidly than thosetreated with the less-polar hexane and methylene chloride, but it was not possible to determine if this was because methanols were more polar or because they were more ethanol-based.

Evolution of worker sterility in honey bees: egg-laying workers express queen-like secretion in Dufour’s gland

It is hypothesized that unlike the normal workers studied by Katzav-Gozansky et al. (1997a, b), anarchistic workers have Dufour's glands that secrete chemicals similar to those from the queen gland, which probably results from a mutation that allows anarchistic laying workers to evade policing.

Worker policing in the honeybee

Experiments showing strong discrimination by honeybee (Apis mellifera) workers against worker-laid male eggs are described, supporting the worker-policing hypothesis.

Worker policing in the bee Apis florea

It is suggested that worker policing via oophagy of worker-laid eggs is pleisiomorphic for the genus A. florea, suggesting that worker sterility in this species is maintained by mutual policing mechanisms that keep worker reproduction at an extremely low level.

Worker policing and worker reproduction in Apis cerana

Workers of the Asian hive bee, Apis cerana, are shown to have relatively high rates of worker ovary activation, and a cerana continue to police worker-laid eggs, even after worker oviposition has commenced in a queenless colony.

Evolution of worker sterility in honey-bees (Apis mellifera): how anarchistic workers evade policing by laying eggs that have low removal rates

The survival of three classes of eggs was compared in both queenright normal colonies and queenright anarchistic colonies to test the hypothesis that anarchistic workers evade policing by laying more acceptable eggs, indicating the egg-removal aspect of the anarchistic syndrome involves both worker laying of eggs with greater acceptability and reduced discriminatory behaviour of policing workers.

The role of queen pheromones in social insects: queen control or queen signal?

It is argued in this paper that pheromonal queen control has never conclusively been demonstrated and is evolutionarily difficult to justify, and a series of experimental and field studies in which positive results would give prima facie evidence for phersomonalQueen control are suggested.

Egg-laying, egg-removal, and ovary development by workers in queenright honey bee colonies

  • F. Ratnieks
  • Biology
    Behavioral Ecology and Sociobiology
  • 2004
Data show that worker egg-laying and worker policing are both normal, though rare, in queenright honey bee colonies, and provide further confirmation of the worker policing hypothesis.

Convergent evolution of worker policing by egg eating in the honeybee and common wasp

  • K. FosterF. Ratnieks
  • Biology
    Proceedings of the Royal Society of London. Series B: Biological Sciences
  • 2001
Worker policing in V.vulgaris may be selected due to the colony–level benefit of conflict suppression, and genetic analysis revealed that workers are equally related to the queen's and other workers' sons.

Reproductive Harmony via Mutual Policing by Workers in Eusocial Hymenoptera

Examination of the cause-effect relationship between queen mating frequency and worker policing indicates that worker policing is caused by queen polyandry but thatworker policing is unlikely to cause polyandries, although it may help stabilize it if police workers show behavioral dominance.