Efficient wheat germ agglutinin purification with a chitosan-based affinity chromatographic matrix.


An efficient affinity chromatographic matrix based on chitosan for wheat germ agglutinin (WGA) purification was developed. The matrices assayed consisted of chitosan mini-spheres cross-linked with epichlorhydrin 45, 250 or 500 mM. The maximum adsorption capacity of pure WGA - calculated from the corresponding isotherms - was between 43.2 and 48.9 mg/g at pH… (More)
DOI: 10.1002/jssc.201100730


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