Efficient water removal in lipase-catalyzed esterifications using a low-boiling-point azeotrope.

@article{Yan2002EfficientWR,
  title={Efficient water removal in lipase-catalyzed esterifications using a low-boiling-point azeotrope.},
  author={Youchun Yan and Uwe T Bornscheuer and Rolf D. Schmid},
  journal={Biotechnology and bioengineering},
  year={2002},
  volume={78 1},
  pages={31-4}
}
High conversions in lipase-catalyzed syntheses of esters from free acyl donors and an alcohol requires efficient removal of water preferentially at temperatures compatible to enzyme activity. Using a lipase B from Candida antarctica (CAL-B)-mediated synthesis of sugar fatty-acid esters, we show that a mixture of ethyl methylketone (EMK) and hexane (best ratio: 4:1, vo/vo) allows efficient removal of water generated during esterification. Azeotropic distillation of the solvent mixture… CONTINUE READING