Efficient uniform isotope labeling of Abl kinase expressed in Baculovirus-infected insect cells.

@article{Strauss2005EfficientUI,
  title={Efficient uniform isotope labeling of Abl kinase expressed in Baculovirus-infected insect cells.},
  author={Andr{\'e} Strauss and Francis Bitsch and Gabriele Fendrich and Patrick Graff and Ren{\'e} Knecht and Bernd Meyhack and Wolfgang Jahnke},
  journal={Journal of biomolecular NMR},
  year={2005},
  volume={31 4},
  pages={
          343-9
        }
}
This report shows for the first time the efficient uniform isotope labeling of a recombinant protein expressed using Baculovirus-infected insect cells. The recent availability of suitable media for (15)N- and (13)C/(15)N-labeling in insect cells, the high expression of Abl kinase in these labeling media and a suitable labeling protocol made it possible to obtain a (1)H-(15)N-HSQC spectrum for the catalytic domain of Abl kinase of good quality and with label incorporation rates > 90%. The… CONTINUE READING
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