Efficient transposition of the Tnt1 tobacco retrotransposon in the model legume Medicago truncatula.

@article{dErfurth2003EfficientTO,
  title={Efficient transposition of the Tnt1 tobacco retrotransposon in the model legume Medicago truncatula.},
  author={Isabelle d'Erfurth and Viviane Cosson and Alexis Eschstruth and H{\'e}l{\`e}ne Lucas and Ad{\'a}m Kondorosi and Pascal Ratet},
  journal={The Plant journal : for cell and molecular biology},
  year={2003},
  volume={34 1},
  pages={95-106}
}
The tobacco element, Tnt1, is one of the few active retrotransposons in plants. Its transposition is activated during protoplast culture in tobacco and tissue culture in the heterologous host Arabidopsis thaliana. Here, we report its transposition in the R108 line of Medicago truncatula during the early steps of the in vitro transformation-regeneration process. Two hundred and twenty-five primary transformants containing Tnt1 were obtained. Among them, 11.2% contained only transposed copies of… CONTINUE READING

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