Efficient transposition of IS911 circles in vitro.

  title={Efficient transposition of IS911 circles in vitro.},
  author={Bao Ton-Hoang and Patrice Polard and Michael Chandler},
  journal={The EMBO journal},
  volume={17 4},
An in vitro system has been developed which supports efficient integration of transposon circles derived from the bacterial insertion sequence IS911. Using relatively pure preparations of IS911-encoded proteins it has been demonstrated that integration into a suitable target required both the transposase, OrfAB, a fusion protein produced by translational frameshifting between two consecutive open reading frames, orfA and orfB, and OrfA, a protein synthesized independently from the upstream orfA… CONTINUE READING
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