Efficient translation destabilizes transcripts in chloroplasts of Chlamydomonas reinhardtii.

  title={Efficient translation destabilizes transcripts in chloroplasts of Chlamydomonas reinhardtii.},
  author={Ko Kato and Kiyohide Ishikura and Seitaro Kasai and Atsuhiko Shinmyō},
  journal={Journal of bioscience and bioengineering},
  volume={101 6},
We previously reported that high level of reporter gene transcript does not confer high amount of reporter protein accumulation in Chlamydomonas reinhardtii chloroplast transformants. Here, to further clarify the correlation between the level of transcript and protein accumulation, we generated the beta-glucuronidase (GUS) reporter gene (uidA) constructs with different potential for translation efficiency of the GUS protein by incorporating different 5' and 3'-untranslated regions of… CONTINUE READING

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