Efficient transformation of Dictyostelium discoideum amoebae.

  title={Efficient transformation of Dictyostelium discoideum amoebae.},
  author={Sheilagh Barclay and Emanuel Meller},
  journal={Molecular and cellular biology},
  volume={3 12},
We have transformed Dictyostelium discoideum amoebae by using derivatives of a plasmid, pAG60, which was designed for transformation of mammalian cells. The plasmid carries the promoter region of the herpes simplex virus type 1 thymidine kinase gene linked to the bacterial gene kan, which codes for the enzyme aminoglycoside 3'-phosphotransferase. kan is… CONTINUE READING