Efficient transduction of primary human B lymphocytes and nondividing myeloma B cells with HIV-1-derived lentiviral vectors.

@article{Bovia2003EfficientTO,
  title={Efficient transduction of primary human B lymphocytes and nondividing myeloma B cells with HIV-1-derived lentiviral vectors.},
  author={Fabrice Bovia and Patrick F. Salmon and Thomas W. Matthes and Kriszti{\'a}n Kvell and Tuan Huy Nguyen and Christiane Werner-Favre and Marc Barnet and M{\'o}nika Nagy and Florence Leuba and Jean-François Arrighi and Vincent Piguet and Didier Trono and Rudolf H. Zubler},
  journal={Blood},
  year={2003},
  volume={101 5},
  pages={
          1727-33
        }
}
We studied the transduction of primary human B lymphocytes and myeloma cells with lentiviral vectors. In peripheral blood B cells that had been activated with helper T cells (murine thymoma EL-4 B5) and cytokines, multiply attenuated HIV-1-derived vectors pseudotyped with vesicular stomatitis virus (VSV) G-envelope protein achieved the expression of green fluorescence protein (GFP) in 27% +/- 12% (mean +/- 1 SD; median, 27%) of B cells in different experiments. When compared in parallel… CONTINUE READING

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