Efficient transduction of monocyte- and CD34+-derived Langerhans cells with lentiviral vectors in the absence of phenotypic and functional maturation.

@article{Vron2006EfficientTO,
  title={Efficient transduction of monocyte- and CD34+-derived Langerhans cells with lentiviral vectors in the absence of phenotypic and functional maturation.},
  author={P. V{\'e}ron and Sylvie Boutin and Jp Bernard and Olivier Danos and Jean M. Davoust and Carole Masurier},
  journal={The journal of gene medicine},
  year={2006},
  volume={8 8},
  pages={951-61}
}
BACKGROUND Gene delivery in dendritic cells (DC) has raised considerable interest to modulate DC functions and induce therapeutic immunity or tolerance in an antigen-specific fashion. Among immature DC, Langerhans cells (LC) are attractive candidates for antigen delivery using lentiviral vectors (LV). METHODS LC derived from monocytes (Mo-LC), or derived from CD34+ cells (CD34-LC) in the presence of cytokine cocktail, were transduced with LV expressing enhanced green fluorescent protein (E… CONTINUE READING