Efficient transcription of the EBER2 gene depends on the structural integrity of the RNA.

@article{Dmpelmann2003EfficientTO,
  title={Efficient transcription of the EBER2 gene depends on the structural integrity of the RNA.},
  author={Edda D{\"u}mpelmann and Hendrik Mittendorf and B J. Benecke},
  journal={RNA},
  year={2003},
  volume={9 4},
  pages={432-42}
}
A 3'-truncated EBER2 RNA gene, although containing all previously identified promoter elements, revealed drastically reduced transcription rates in vitro and in vivo when fused to a heterologous terminator sequence. Inactivations were also observed with double point mutations affecting 5'- or 3'-end sequences of the EBER2 gene. However, wild-type activity of these mutants could be restored by compensatory mutations of the opposite strand of the EBER2 RNA sequence. A similar rescue was achieved… CONTINUE READING