Efficient synthetic inhibitors of anthrax lethal factor.

Abstract

Inhalation anthrax is a deadly disease for which there is currently no effective treatment. Bacillus anthracis lethal factor (LF) metalloproteinase is an integral component of the tripartite anthrax lethal toxin that is essential for the onset and progression of anthrax. We report here on a fragment-based approach that allowed us to develop inhibitors of LF. The small-molecule inhibitors we have designed, synthesized, and tested are highly potent and selective against LF in both in vitro tests and cell-based assays. These inhibitors do not affect the prototype human metalloproteinases that are structurally similar to LF. Initial in vivo evaluation of postexposure efficacy of our inhibitors combined with antibiotic ciprofloxacin against B. anthracis resulted in significant protection. Our data strongly indicate that the scaffold of inhibitors we have identified is the foundation for the development of novel, safe, and effective emergency therapy of postexposure inhalation anthrax.

6 Figures and Tables

050100150'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

395 Citations

Semantic Scholar estimates that this publication has 395 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Forino2005EfficientSI, title={Efficient synthetic inhibitors of anthrax lethal factor.}, author={Martino Forino and Sherida L Johnson and Thiang Yian Wong and Dmitri V . Rozanov and Alexei Y Savinov and Wei Li and Roberto Fattorusso and Barbara Becattini and Andrew J W Orry and Dawoon Jung and Ruben Abagyan and Jeffrey W. Smith and Ken Alibek and Robert C . Liddington and Alex Y . Strongin and Maurizio Pellecchia}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={2005}, volume={102 27}, pages={9499-504} }