Efficient strategy for the rapid backbone assignment of membrane proteins.

@article{Trbovic2005EfficientSF,
  title={Efficient strategy for the rapid backbone assignment of membrane proteins.},
  author={Nikola Trbovic and Christian Klammt and Alexander Koglin and Frank Loehr and Frank Bernhard and Volker D{\"o}tsch},
  journal={Journal of the American Chemical Society},
  year={2005},
  volume={127 39},
  pages={13504-5}
}
Investigations of membrane proteins pose one of the biggest current challenges in structural biology. Recent advances in protein production techniques based on cell-free transcription/translation methods have, however, opened new opportunities in this area. Here, we report an efficient protocol for the backbone assignment of membrane proteins as the first step of NMR-based structure determination. 

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