Efficient strategy for the rapid backbone assignment of membrane proteins.

Abstract

Investigations of membrane proteins pose one of the biggest current challenges in structural biology. Recent advances in protein production techniques based on cell-free transcription/translation methods have, however, opened new opportunities in this area. Here, we report an efficient protocol for the backbone assignment of membrane proteins as the first step of NMR-based structure determination.

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@article{Trbovic2005EfficientSF, title={Efficient strategy for the rapid backbone assignment of membrane proteins.}, author={Nikola Trbovic and Christian Klammt and Alexander Koglin and Frank Loehr and Frank Bernhard and Volker D{\"{o}tsch}, journal={Journal of the American Chemical Society}, year={2005}, volume={127 39}, pages={13504-5} }