Efficient soluble protein production on transgenic silkworms expressing cytoplasmic chaperones

@article{Hong2010EfficientSP,
  title={Efficient soluble protein production on transgenic silkworms expressing cytoplasmic chaperones},
  author={Sun Mee Hong and Jun Yamashita and Hitoshi Mitsunobu and Keiro Uchino and Isao Kobayashi and Hideki Sezutsu and Toshiki Tamura and Hideki Nakajima and Yoshitaka Miyagawa and Jae Man Lee and Hiroaki Mon and Yoshihiko Miyata and Yutaka Kawaguchi and Takahiro Kusakabe},
  journal={Applied Microbiology and Biotechnology},
  year={2010},
  volume={87},
  pages={2147-2156}
}
Baculovirus expression systems (BES) are widely used for recombinant protein production in lepidopteran cells or larvae. However, even in BES, the insolubility of recombinant proteins sometimes makes their expression difficult. In this study, to improve the solubility and yield of foreign proteins, we constructed transgenic silkworms using silkworm heat-shock proteins, Hsp70 and Hsp40, or Hsc70 and Hsp90 co-chaperone Hop. In these transgenic silkworms, the expression levels of the transgenes… CONTINUE READING