Efficient site-directed in vitro mutagenesis using phagemid vectors.

@article{McClary1989EfficientSI,
  title={Efficient site-directed in vitro mutagenesis using phagemid vectors.},
  author={J. A. McClary and Frank R. Witney and J. Geisselsoder},
  journal={BioTechniques},
  year={1989},
  volume={7 3},
  pages={282-9}
}
Several methods have been developed that enhance the efficiency of in vitro, site-directed mutagenesis. Kunkel (8,9) has developed a method which uses a strong selection for the mutated strand and, hence, is highly efficient, but yet simple and rapid. This method originally used M13 phage as the vector. In this paper, we describe a refinement of this method using phagemid vectors, which combine the advantages of plasmids (such as high copy number and stability of cloned DNA) with the single… CONTINUE READING

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