Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting.

@article{Borth2000EfficientSO,
  title={Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting.},
  author={Nicole Borth and Maximilian Zeyda and Renate E Kunert and Hermann Katinger},
  journal={Biotechnology and bioengineering},
  year={2000},
  volume={71 4},
  pages={266-73}
}
The screening procedure for high-producing cell lines is extremely time- and labor-intensive and costly, and is at present guided by an empirical approach based on individual experience. Flow cytometry and cell sorting, with its ability to analyze and separate single cells, an ideal method in the selection of such rare cells. The isolation of recombinant cell lines is especially difficult due to repeated gene amplification, which introduces high mutational variation into the population. We have… CONTINUE READING

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