Efficient resonance assignment of proteins in MAS NMR by simultaneous intra- and inter-residue 3D correlation spectroscopy

@article{Daviso2013EfficientRA,
  title={Efficient resonance assignment of proteins in MAS NMR by simultaneous intra- and inter-residue 3D correlation spectroscopy},
  author={Eugenio Daviso and Matthew T Eddy and Loren B Andreas and Robert G Griffin and Judith Herzfeld},
  journal={Journal of Biomolecular NMR},
  year={2013},
  volume={55},
  pages={257-265}
}
Resonance assignment is the first step in NMR structure determination. For magic angle spinning NMR, this is typically achieved with a set of heteronuclear correlation experiments (NCaCX, NCOCX, CONCa) that utilize SPECIFIC-CP 15N–13C transfers. However, the SPECIFIC-CP transfer efficiency is often compromised by molecular dynamics and probe performance. Here we show that one-bond ZF-TEDOR 15N–13C transfers provide simultaneous NCO and NCa correlations with at least as much sensitivity as… CONTINUE READING

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