Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells.

@article{Nagata2009EfficientRO,
  title={Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells.},
  author={Shogo Nagata and Masashi Toyoda and Shinpei Yamaguchi and Kunio Hirano and Hatsune Makino and Koichiro Nishino and Yoshitaka Miyagawa and Hajime Okita and Nobutaka Kiyokawa and Masato Nakagawa and Shinya Yamanaka and Hidenori Akutsu and Akihiro Umezawa and Takashi Tada},
  journal={Genes to cells : devoted to molecular & cellular mechanisms},
  year={2009},
  volume={14 12},
  pages={
          1395-404
        }
}
Practical clinical applications for current induced pluripotent stem cell (iPSC) technologies are hindered by very low generation efficiencies. Here, we demonstrate that newborn human (h) and mouse (m) extra-embryonic amnion (AM) and yolk-sac (YS) cells, in which endogenous KLF4/Klf4, c-MYC/c-Myc and RONIN/Ronin are expressed, can be reprogrammed to hiPSCs and miPSCs with efficiencies for AM cells of 0.02% and 0.1%, respectively. Both hiPSC and miPSCs are indistinguishable from embryonic stem… CONTINUE READING

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