Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells.

Abstract

Practical clinical applications for current induced pluripotent stem cell (iPSC) technologies are hindered by very low generation efficiencies. Here, we demonstrate that newborn human (h) and mouse (m) extra-embryonic amnion (AM) and yolk-sac (YS) cells, in which endogenous KLF4/Klf4, c-MYC/c-Myc and RONIN/Ronin are expressed, can be reprogrammed to hiPSCs and miPSCs with efficiencies for AM cells of 0.02% and 0.1%, respectively. Both hiPSC and miPSCs are indistinguishable from embryonic stem cells in colony morphology, expression of pluripotency markers, global gene expression profile, DNA methylation status of OCT4 and NANOG, teratoma formation and, in the case of miPSCs, generation of germline transmissible chimeric mice. As copious amounts of human AM cells can be collected without invasion, and stored long term by conventional means without requirement for in vitro culture, they represent an ideal source for cell banking and subsequent 'on demand' generation of hiPSCs for personal regenerative and pharmaceutical applications.

DOI: 10.1111/j.1365-2443.2009.01356.x
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@article{Nagata2009EfficientRO, title={Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells.}, author={Shogo Nagata and Masashi Toyoda and Shinpei Yamaguchi and Kunio Hirano and Hatsune Makino and Koichiro Nishino and Yoshitaka Miyagawa and Hajime Okita and Nobutaka Kiyokawa and Masato Nakagawa and Shinya Yamanaka and Hidenori Akutsu and Akihiro Umezawa and Takashi Tada}, journal={Genes to cells : devoted to molecular & cellular mechanisms}, year={2009}, volume={14 12}, pages={1395-404} }