Efficient removal of PCR inhibitors using agarose-embedded DNA preparations

@article{Moreira1998EfficientRO,
  title={Efficient removal of PCR inhibitors using agarose-embedded DNA preparations},
  author={David Moreira},
  journal={Nucleic acids research},
  year={1998},
  volume={26 13},
  pages={3309-10}
}
The use of agarose blocks containing embedded DNA improves the PCR amplification from templates naturally contaminated with polysaccharides or humic acids, two powerful PCR inhibitors. Presumably, the difference in size between the DNA macromolecules and these contaminants allows their effective removal from the agarose blocks by diffusion during the washing steps, whereas genomic DNA remains trapped within them. In addition, agarose-embedded DNA can be directly used for PCR since low melting… CONTINUE READING