Efficient regeneration of plantlets from callus and mesophyll derived protoplasts of Robinia pseudoacacia L.

@article{Kanwar2008EfficientRO,
  title={Efficient regeneration of plantlets from callus and mesophyll derived protoplasts of Robinia pseudoacacia L.},
  author={Kamlesh Kanwar and Anju Bhardwaj and R. Charu Deepika},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2008},
  volume={96},
  pages={95-103}
}
A protocol for plant regeneration from mesophyll and callus protoplasts of Robinia pseudoacacia L. was developed. For leaves from in vitro raised shoots, an enzyme combination of 2.0% cellulose and 0.3% macerozyme for a digestion period of 20 h resulted in the best yield of protoplasts (9.45 × 105 protoplast/g fresh weight). Mesophyll-derived protoplasts started cell wall regeneration within 24 h of being embedded in Nagata and Takebe (NT) medium supplemented with 5 μM NAA and 1 μM BAP followed… CONTINUE READING
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