Efficient protoplast regeneration for some homofermentative lactobacilli and pediococci

@article{Tanaka2001EfficientPR,
  title={Efficient protoplast regeneration for some homofermentative lactobacilli and pediococci},
  author={Osamu Tanaka and Sadahiro Ohmomo},
  journal={Archives of Microbiology},
  year={2001},
  volume={177},
  pages={36-40}
}
Conditions for protoplast regeneration were examined for several strains of homofermentative lactobacilli and pediococci isolated from silage. Attempts to regenerate protoplasts using previously published agar regeneration media for lactobacilli were unsuccessful for most of the strains. Replacing or increasing colloidal substances in a medium containing raffinose and MgCl2 as osmotic stabilizers enabled efficient regeneration of the protoplasts at a frequency of 10–99%. A medium containing… CONTINUE READING
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