Efficient production of transgenic cattle by retroviral infection of early embryos.

@article{Haskell1995EfficientPO,
  title={Efficient production of transgenic cattle by retroviral infection of early embryos.},
  author={R E Haskell and Richard A. Bowen},
  journal={Molecular reproduction and development},
  year={1995},
  volume={40 3},
  pages={386-90}
}
Production of transgenic cattle by microinjection of DNA has been difficult and costly. To explore an alternative method, one- to four-cell bovine embryos were exposed to a replication-defective retrovirus by microinjection of retrovirus producer cells into the perivitelline space. Embryos were cultured in vitro for 3-4 days, then transferred to recipient… CONTINUE READING