Efficient production of mammalian RNA tumor viruses in serum-free culture medium allows rapid RNA subunit purification.

@article{Strauss1980EfficientPO,
  title={Efficient production of mammalian RNA tumor viruses in serum-free culture medium allows rapid RNA subunit purification.},
  author={Edna Strauss and M Celeste Simon and P. C. Kimball},
  journal={Journal of virological methods},
  year={1980},
  volume={1 4},
  pages={213-21}
}
A wide variety of infected mammalian cell cultures were observed to produce high levels of RNA tumor virus particles in the absence of serum for at least 12 h. Virus production was measured by yields of 50-70 S virus RNAs isolated directly from serum-free culture media by chromatography on oligo(dT)-cellulose. Yields of RNAs from viruses produced in serum… CONTINUE READING