Efficient production of infectious hepatitis C virus with adaptive mutations in cultured hepatoma cells.

@article{Bungyoku2009EfficientPO,
  title={Efficient production of infectious hepatitis C virus with adaptive mutations in cultured hepatoma cells.},
  author={Yasuaki Bungyoku and Ikuo Shoji and Tatsuhiko Makine and Tetsuya Adachi and Kazumi Hayashida and Motoko Nagano-Fujii and Yoshi-hiro Ide and Lin Deng and Hak Hotta},
  journal={The Journal of general virology},
  year={2009},
  volume={90 Pt 7},
  pages={1681-91}
}
Robust production of infectious hepatitis C virus (HCV) in cell culture was realized by using the JFH1 strain and the homologous chimeric J6/JFH1 strain in Huh-7.5 cells, a highly HCV-permissive subclone of Huh-7 cells. In this study, we aimed to establish a more efficient HCV-production system and to gain some insight into the adaptation mechanisms of efficient HCV production. By serial passaging of J6/JFH1-infected Huh-7.5 cells, we obtained culture-adapted J6/JFH1 variants, designated P-27… CONTINUE READING
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