Efficient production of L-asparaginase from Bacillus licheniformis with low-glutaminase activity: optimization, scale up and acrylamide degradation studies.

@article{Mahajan2012EfficientPO,
  title={Efficient production of L-asparaginase from Bacillus licheniformis with low-glutaminase activity: optimization, scale up and acrylamide degradation studies.},
  author={Richi V. Mahajan and Saurabh Saran and Karthikeya Kameswaran and Vinod Kumar and Rajendra Kumar Saxena},
  journal={Bioresource technology},
  year={2012},
  volume={125},
  pages={11-6}
}
L-Asparaginase has potential as an anti-cancer drug and for prevention of acrylamide formation in fried and baked foods. Production of the enzyme by Bacillus licheniformis (RAM-8) was optimized by process engineering using a statistical modeling approach and a maximum yield of 32.26 IU/ml was achieved. The L-asparaginase exhibited glutaminase activity of only 0.8 IU/ml and would therefore be less prone to cause the side effects associated with asparaginase therapy compared to enzyme… CONTINUE READING