Efficient production of Agrobacterium rhizogenes-transformed roots and composite plants for studying gene expression in coffee roots

@article{Alpizar2006EfficientPO,
  title={Efficient production of Agrobacterium rhizogenes-transformed roots and composite plants for studying gene expression in coffee roots},
  author={Edgardo Alpizar and Eveline D{\'e}champ and Sandra Esp{\'e}out and M et Mme Royer and A. C. Lecouls and Michel R. Nicole and Bern{\'o}it Bertrand and Philippe Lashermes and Herv{\'e} Etienne},
  journal={Plant Cell Reports},
  year={2006},
  volume={25},
  pages={959-967}
}
The possibility of rapid validation and functional analysis of nematode resistance genes is a common objective for numerous species and particularly for woody species. In this aim, we developed an Agrobacterium rhizogenes-mediated transformation protocol for Coffea arabica enabling efficient and rapid regeneration of transformed roots from the hypocotyls of germinated zygotic embryos, and the subsequent production of composite plants. The A. rhizogenes strain A4RS proved to be the most virulent… CONTINUE READING

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