Efficient prefractionation of low-abundance proteins in human plasma and construction of a two-dimensional map.

@article{Cho2005EfficientPO,
  title={Efficient prefractionation of low-abundance proteins in human plasma and construction of a two-dimensional map.},
  author={Sang Yun Cho and Eun-young Lee and Joon Seok Lee and Hye-Young Kim and Jae Myun Park and Min-Seok Kwon and Young-Kew Park and Hyoung-Joo Lee and M. -J. Kang and Jin Young Kim and Jong Shin Yoo and Sung Jin Park and Jin Won Cho and Hyon-Suk Kim and Young-Ki Paik},
  journal={Proteomics},
  year={2005},
  volume={5 13},
  pages={
          3386-96
        }
}
Human plasma is the most clinically valuable specimen, containing not only a dynamic concentration range of protein components, but also several groups of high-abundance proteins that seriously interfere with the detection of low-abundance potential biomarker proteins. To establish a high-throughput method for efficient depletion of high-abundance proteins and subsequent fractionation, prior to molecular analysis of proteins, we explored how coupled immunoaffinity columns, commercially… CONTINUE READING
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