Efficient plant regeneration from protoplasts of Kalanchoe blossfeldiana via organogenesis

Abstract

A simple and efficient protocol for plant regeneration from protoplasts of the potted plant Kalanchoe blossfeldiana Poelln. is reported. Mesophyll protoplasts were isolated from axenic leaves after a preculture. The enzymatic digestion of the tissue with a solution containing 0.4% Cellulase Onozuka R-10 and 0.2% Driselase yielded 6.0 × 105 protoplasts per… (More)
DOI: 10.1007/s11240-009-9617-8

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