Efficient pH-independent sequence-specific DNA binding by pseudoisocytosine-containing bis-PNA.

  title={Efficient pH-independent sequence-specific DNA binding by pseudoisocytosine-containing bis-PNA.},
  author={Michael Egholm and Leif Christensen and K L Dueholm and O. Buchardt and James Coull and Peter E. Nielsen},
  journal={Nucleic acids research},
  volume={23 2},
The synthesis and DNA binding properties of bis-PNA (peptide nucleic acid) are reported. Two PNA segments each of seven nucleobases in length were connected in a continuous synthesis via a flexible linker composed of three 8-amino-3,6-dioxaoctanoic acid units. The sequence of the first strand was TCTCTTT (C- to N-terminal), while the second strand was TTTCTCT or TTTJTJT, where J is pseudoisocytosine. These bis-PNAs form triple-stranded complexes of somewhat higher thermal stability than… CONTINUE READING

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Biochemistry 9, 3136-3142

M. Egholm, O. Buchardt, P. E. Nielsen, R. H. Berg
J. Am. Chem.Soc • 1992

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