Efficient oxygen transfer by surface aeration in shaken cylindrical containers for mammalian cell cultivation at volumetric scales up to 1000 L

@inproceedings{Zhang2009EfficientOT,
  title={Efficient oxygen transfer by surface aeration in shaken cylindrical containers for mammalian cell cultivation at volumetric scales up to 1000 L},
  author={Xiaowei Zhang and C{\'e}dric-Alain B{\"u}rki and Matthieu Stettler and Dario De Sanctis and Marco Perrone and Marco Discacciati and Nicola Parolini and M. Cristina B. Dejesus and D Hacker and Alfio Quarteroni and F. M. Wurm},
  year={2009}
}
Cylindrical containers agitated by orbital shaking are being developed as simple and cost-effective bioreactor systems for the cultivation of mammalian cells. Here the oxygen transfer capacities of containers with nominal volumes from 50 mL to 2000 L were determined, and the operating parameters influencing oxygen transfer were investigated. In general, the shaking speed necessary for efficient oxygen transfer diminished as the container size increased. At shaking speeds suitable for the growth… CONTINUE READING

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