Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells.

Abstract

Transgenic and gene-targeted mutant mice provide powerful tools for analysis of the cellular processes involved in early development and in the pathogenesis of many diseases. Here we describe a transgene integration strategy mediated by site-specific recombination that allows establishment of multiple embryonic stem (ES) cell lines carrying tetracycline… (More)

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@article{Beard2006EfficientMT, title={Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells.}, author={Caroline F Beard and Konrad Hochedlinger and Kathrin Plath and Anton Wutz and Rudolf Jaenisch}, journal={Genesis}, year={2006}, volume={44 1}, pages={23-8} }