Efficient isolation of genes by using antibody probes.

  title={Efficient isolation of genes by using antibody probes.},
  author={Richard A. Young and Ronald W. Davis},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={80 5},
A sensitive and general technique has been devised for the dual purposes of cloning genes by using antibodies as probes and isolating unknown proteins encoded by cloned DNA. The method uses an expression vector, lambda gt11 (lac5 nin5 cI857 S100), that permits insertion of foreign DNA into the beta-galactosidase structural gene lacZ and promotes synthesis of hybrid proteins. Efficient screening of antigen-producing clones in lambda gt11 recombinant cDNA libraries is achieved through lysogeny of… CONTINUE READING

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