Efficient isolation and long-term viability of bovine small preantral follicles in vitro

@article{Itoh2000EfficientIA,
  title={Efficient isolation and long-term viability of bovine small preantral follicles in vitro},
  author={Takehiro Itoh and Hiroyoshi Hoshi},
  journal={In Vitro Cellular & Developmental Biology - Animal},
  year={2000},
  volume={36},
  pages={235-240}
}
A comparison of isolation techniques for small preantral follicles (30–70 μm) from bovine ovaries using a mechanical method with a grating device or collagenase treatment was performed. The mean number (157.0) of intact follicles per ovary isolated by the mechanical method was significantly greater (P<0.05) than that (26.0) of follicles isolated by the enzymatic method. Isolated morphologically normal follicles (MNF) were cultured for up to 30 d either in control cultures (non-coculture) or in… CONTINUE READING

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