Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9

@article{Paquet2016EfficientIO,
  title={Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9},
  author={Dominik Paquet and Dylan Kwart and Antonia F. Chen and Andrew A. Sproul and Samson T. Jacob and Shaun S.K. Teo and K M Olsen and Andrew Gregg and Scott A. Noggle and Marc Tessier-Lavigne},
  journal={Nature},
  year={2016},
  volume={533},
  pages={125-129}
}
  • Dominik Paquet, Dylan Kwart, +7 authors Marc Tessier-Lavigne
  • Published in Nature 2016
  • Medicine, Biology
  • The bacterial CRISPR/Cas9 system allows sequence-specific gene editing in many organisms and holds promise as a tool to generate models of human diseases, for example, in human pluripotent stem cells. CRISPR/Cas9 introduces targeted double-stranded breaks (DSBs) with high efficiency, which are typically repaired by non-homologous end-joining (NHEJ) resulting in nonspecific insertions, deletions or other mutations (indels). DSBs may also be repaired by homology-directed repair (HDR) using a DNA… CONTINUE READING

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