Efficient initiation of mammalian mRNA translation at a CUG codon.

  title={Efficient initiation of mammalian mRNA translation at a CUG codon.},
  author={M C Dasso and Richard J. Jackson},
  journal={Nucleic acids research},
  volume={17 16},
Nucleotide substitutions were made at the initiation codon of an influenza virus NS cDNA clone in a vector carrying the bacteriophage T7 promoter. When capped mRNA transcripts of these constructs were translated in the rabbit reticulocyte lysate, a change in the initiation codon from...AUAAUGG...to...AUACUGG...reduced the in vitro translational efficiency by only 50-60%, and resulted in only a small increase in the yield of short products presumed to be initiated at downstream sites. Synthesis… CONTINUE READING


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The Origin of Pandemic Influenza Viruses, ed

  • J. F. Young, U. Desselberger, P. Graves, P. Palese, A. Shatzman, M. Rosenberg
  • 1983

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