Efficient infection of primary tupaia hepatocytes with purified human and woolly monkey hepatitis B virus.

@article{Kck2001EfficientIO,
  title={Efficient infection of primary tupaia hepatocytes with purified human and woolly monkey hepatitis B virus.},
  author={Josef K{\"o}ck and Michael Nassal and Sabine MacNelly and Thomas F Baumert and H. E. Blum and Fritz von Weizs{\"a}cker},
  journal={Journal of virology},
  year={2001},
  volume={75 11},
  pages={5084-9}
}
The Asian tree shrew, Tupaia belangeri, has been proposed as a novel animal model for studying hepatitis B virus (HBV) infection. Here, we describe a protocol for efficient and reproducible infection of primary tupaia hepatocytes with HBV. We report that human serum interferes with HBV binding to the hepatocytes, thus limiting the maximum multiplicity of infection. Purification of HBV virions by gradient sedimentation greatly enhances virus binding and infectivity. Covalently closed circular… CONTINUE READING