Efficient infection of a human T-cell line and of human primary peripheral blood leukocytes with a pseudotyped retrovirus vector.

@article{Sharma1996EfficientIO,
  title={Efficient infection of a human T-cell line and of human primary peripheral blood leukocytes with a pseudotyped retrovirus vector.},
  author={Shilpi Sharma and Mark J. Cantwell and Thomas J. Kipps and Theodore Friedmann},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 21},
  pages={11842-7}
}
Peripheral blood lymphocytes (PBLs) are an important target for gene transfer studies aimed at human gene therapy. However, no reproducibly efficient methods are currently available to transfer foreign, potentially therapeutic genes into these cells. While vectors derived from murine retroviruses have been the most widely used system, their low infection efficiency in lymphocytes has required prolonged in vitro culturing and selection after infection to obtain useful numbers of genetically… CONTINUE READING
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