Efficient incorporation of deuterated amino acids into quail egg white proteins for nuclear magnetic resonance studies.

@article{BrownMason1981EfficientIO,
  title={Efficient incorporation of deuterated amino acids into quail egg white proteins for nuclear magnetic resonance studies.},
  author={A S Brown-Mason and Christopher M. Dobson and Robert C. Woodworth},
  journal={The Journal of biological chemistry},
  year={1981},
  volume={256 4},
  pages={
          1506-9
        }
}
The in vivo incorporation of deuterated amino acids into egg white proteins of Japanese quail is described. Using a synthetic diet, the level of incorporation of selectively deuterated tyrosine, tryptophan, histidine, and phenylalanine into lysozyme was greater than 80% as demonstrated by proton NMR. Load and load-chase experiments using [3H]phenylalanine or [3H]leucine monitored the fast uptake time (t 1/2 = 2 days) and confirmed the high levels of incorporation. The potential of this system… CONTINUE READING

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