Efficient human hematopoietic cell transduction using RD114- and GALV-pseudotyped retroviral vectors produced in suspension and serum-free media.

@article{Ghani2009EfficientHH,
  title={Efficient human hematopoietic cell transduction using RD114- and GALV-pseudotyped retroviral vectors produced in suspension and serum-free media.},
  author={K. Ghani and X. Wang and P. O. de Campos-Lima and M. Olszewska and A. Kamen and I. Rivi{\'e}re and M. Caruso},
  journal={Human gene therapy},
  year={2009},
  volume={20 9},
  pages={
          966-74
        }
}
  • K. Ghani, X. Wang, +4 authors M. Caruso
  • Published 2009
  • Biology, Medicine
  • Human gene therapy
  • Retroviral vectors derived from the Moloney murine leukemia virus have been used in successful and promising gene therapy clinical trials. However, platforms for their large-scale production must be further developed. As a proof of principle, we reported the generation of a packaging cell line that produces amphotropic retroviral vectors in suspension and serum-free medium (SFM). In the present study, we have constructed and characterized two retroviral packaging cell lines designed for gene… CONTINUE READING
    32 Citations
    Generation of High-Titer Self-Inactivated γ-Retroviral Vector Producer Cells
    • 1
    Large-scale Clinical-grade Retroviral Vector Production in a Fixed-Bed Bioreactor
    • 40
    GMP-Grade Manufacturing of T Cells Engineered to Express a Defined γδTCR
    • 19
    • Highly Influenced
    • PDF
    CAR therapy : the CD 19 paradigm
    • 20

    References

    SHOWING 1-10 OF 46 REFERENCES
    Gene transfer to repopulating human CD34+ cells using amphotropic-, GALV-, or RD114-pseudotyped HIV-1-based vectors from stable producer cells.
    • 46
    Generation of a high-titer packaging cell line for the production of retroviral vectors in suspension and serum-free media
    • 39
    • PDF
    Inducible packaging cells for large-scale production of lentiviral vectors in serum-free suspension culture.
    • 116
    Characterization of HIV-1 vectors with gammaretrovirus envelope glycoproteins produced from stable packaging cells
    • 71
    • PDF
    Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus.
    • 606
    • PDF
    Retroviral vector production using suspension‐adapted 293GPG cells in a 3L acoustic filter‐based perfusion bioreactor
    • 26
    Analysis of 4070A envelope levels in retroviral preparations and effect on target cell transduction efficiency.
    • 37