Efficient human hematopoietic cell transduction using RD114- and GALV-pseudotyped retroviral vectors produced in suspension and serum-free media.

@article{Ghani2009EfficientHH,
  title={Efficient human hematopoietic cell transduction using RD114- and GALV-pseudotyped retroviral vectors produced in suspension and serum-free media.},
  author={Karim Ghani and Xiuyan Wang and Pedro Otavio de Campos-Lima and Malgorzata Olszewska and Amine A Kamen and Isabelle Rivi{\'e}re and Manuel Caruso},
  journal={Human gene therapy},
  year={2009},
  volume={20 9},
  pages={
          966-74
        }
}
Retroviral vectors derived from the Moloney murine leukemia virus have been used in successful and promising gene therapy clinical trials. However, platforms for their large-scale production must be further developed. As a proof of principle, we reported the generation of a packaging cell line that produces amphotropic retroviral vectors in suspension and serum-free medium (SFM). In the present study, we have constructed and characterized two retroviral packaging cell lines designed for gene… CONTINUE READING

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