Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects

  title={Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects},
  author={Bin Shen and Wensheng Zhang and Jun Zhang and Jiankui Zhou and Jianying Wang and Li Chen and Lu Wang and Alex Hodgkins and Vivek Iyer and Xingxu Huang and William C. Skarnes},
  journal={Nature Methods},
Bacterial RNA–directed Cas9 endonuclease is a versatile tool for site-specific genome modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutations that are transmissible to offspring. However, Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for genome editing of any model organism and minimizes confounding problems of off-target… CONTINUE READING
Highly Influential
This paper has highly influenced 10 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
Related Discussions
This paper has been referenced on Twitter 10 times. VIEW TWEETS

From This Paper

Figures, tables, and topics from this paper.


Publications citing this paper.
Showing 1-10 of 202 extracted citations

Mouse Genome Editing Using the CRISPR/Cas System.

Current protocols in human genetics • 2014
View 8 Excerpts
Highly Influenced

Initiation of homologous recombination at DNA nicks

Nucleic acids research • 2018
View 4 Excerpts
Highly Influenced

Efficient genome editing of differentiated renal epithelial cells

Pflügers Archiv - European Journal of Physiology • 2016
View 10 Excerpts
Highly Influenced

CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes

Protein & Cell • 2015
View 3 Excerpts
Highly Influenced

Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas9

Journal of molecular cell biology • 2015
View 5 Excerpts
Highly Influenced


Publications referenced by this paper.

N at ur e A m er ic a, In c. A ll rig

A. R. Quinlan

Similar Papers

Loading similar papers…