Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects

@article{Shen2014EfficientGM,
  title={Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects},
  author={Bin Shen and Wensheng Zhang and Jun Zhang and Jiankui Zhou and Jianying Wang and Li Chen and Lu Wang and Alex Hodgkins and Vivek Iyer and Xingxu Huang and William C. Skarnes},
  journal={Nature Methods},
  year={2014},
  volume={11},
  pages={399-402}
}
Bacterial RNA–directed Cas9 endonuclease is a versatile tool for site-specific genome modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutations that are transmissible to offspring. However, Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for genome editing of any model organism and minimizes confounding problems of off-target… CONTINUE READING
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N at ur e A m er ic a, In c. A ll rig

A. R. Quinlan
2010

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