Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange.

  title={Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange.},
  author={Aran F Labrijn and Joyce I Meesters and Bart E C G de Goeij and Ewald T J van den Bremer and Joost J. Neijssen and Muriel D. van Kampen and Kristin Strumane and Sandra Verploegen and Amitava Kundu and Michael J. Gramer and Patrick H.C. van Berkel and Jan G. J. van de Winkel and Janine Schuurman and Paul W H I Parren},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={110 13},
The promise of bispecific antibodies (bsAbs) to yield more effective therapeutics is well recognized; however, the generation of bsAbs in a practical and cost-effective manner has been a formidable challenge. Here we present a technology for the efficient generation of bsAbs with normal IgG structures that is amenable to both antibody drug discovery and development. The process involves separate expression of two parental antibodies, each containing single matched point mutations in the CH3… CONTINUE READING


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