Efficient generation of goats with defined point mutation (I397V) in GDF9 through CRISPR/Cas9.

@article{Niu2018EfficientGO,
  title={Efficient generation of goats with defined point mutation (I397V) in GDF9 through CRISPR/Cas9.},
  author={Yiyuan Niu and Xiaoe Zhao and Jiankui Zhou and Yan Li and Yu Ming Huang and Bei Cai and Yutai Liu and Qiang Ding and Shiwei Zhou and Jin Zhao and Guangxian Zhou and Baohua Ma and Xingxu Huang and Xiaolong Wang and Yulin Chen},
  journal={Reproduction, fertility, and development},
  year={2018},
  volume={30 2},
  pages={
          307-312
        }
}
The recent emergence of the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) 9 system has attracted significant attention for its potential to improve traits of agricultural importance. However, most applications in livestock species to date have depended on aberrant DNA repair to generate frameshifting indels. Whether this genomic engineering technique involving homology-dependent repair (HDR) can be used to introduce defined point mutations has been… CONTINUE READING