Efficient generation of antibodies to oncoproteins by using synthetic peptide antigens.

@article{Tanaka1985EfficientGO,
  title={Efficient generation of antibodies to oncoproteins by using synthetic peptide antigens.},
  author={Teruo Tanaka and Dennis J. Slamon and Martin J. Cline},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1985},
  volume={82 10},
  pages={3400-4}
}
To examine the efficiency of generating protein-reactive antipeptide antibodies, 35 peptides encoded by retroviral or cellular oncogenes were used to immunize rabbits. Thirty-two peptides elicited antipeptide antibodies, of which 56% reacted with their respective oncoproteins. The length of the immunizing peptide was an important factor in generating antibodies reactive with native protein. Similar peptides differing in a single or a few amino acids could elicite antisera of markedly different… CONTINUE READING

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