Efficient gene editing via non-viral delivery of CRISPR-Cas9 system using polymeric and hybrid microcarriers.

@article{Timin2018EfficientGE,
  title={Efficient gene editing via non-viral delivery of CRISPR-Cas9 system using polymeric and hybrid microcarriers.},
  author={Alexander S Timin and Albert Radikovich Muslimov and Kirill V. Lepik and Olga S. Epifanovskaya and Alena I. Shakirova and Ulrike Mock and Kristoffer Riecken and Maria V. Okilova and Vladislav S. Sergeev and Boris Vladimirovich Afanasyev and Boris Fehse and Gleb B. Sukhorukov},
  journal={Nanomedicine : nanotechnology, biology, and medicine},
  year={2018},
  volume={14 1},
  pages={
          97-108
        }
}
CRISPR-Cas9 is a revolutionary genome-editing technology that has enormous potential for the treatment of genetic diseases. However, the lack of efficient and safe, non-viral delivery systems has hindered its clinical application. Here, we report on the application of polymeric and hybrid microcarriers, made of degradable polymers such as polypeptides and polysaccharides and modified by silica shell, for delivery of all CRISPR-Cas9 components. We found that these microcarriers mediate more… CONTINUE READING
BETA